I received my undergraduate
education at the Moscow Institute of Physics
and Technology, graduating in 1987. I have mastered molecular
biological and biochemical techniques working one year at the Student
Center of the Shemyakin's Institute
of Bioorganic Chemistry. My M.S. thesis was carried out in the lab
of professor N.N.
Modyanov. After one year of working as a research Associate in
the professor Modyanov's lab I immigrated with my family to the United
States.
In USA, Dr.
Joseph
M. Ahearn at Johns Hopkins University hired me as a technician.
After one year of working there we have published a paper and I entered
the Graduate Program in Biochemistry, Cellular and Molecular Biology at
Johns Hopkins University, School of Medicine. My rotation project
in Daniel Nathans
laboratory was to identify peptide with in vivo phosphorylation sites of
JunB protein by its phosphopeptide analysis.
I received my Ph.D.
under Dr. Jeffry
L. Corden, studying the function of the C-terminal domain (CTD) of
eukaryotic RNA-polymerase II. My Ph.D. thesis consists of two parts.
First, I used yeast genetics to study CTD phosphorylation site suppressor
mutants. I have shown that two different phosphorylation sites on
the CTD can be genetically distinguished by suppression analysis.
As a second project I used the two-hybrid method to clone genes encoding
proteins that interact with the CTD. The bioinformatics analysis
of CTD interacting proteins yielded a discovery of novel splicing-related
proteins interacting with RNA polymerase II. The discovery of these
proteins suggested the existence of the physical link between transcription
and splicing as well as a novel mechanism of gene expression regulation.
I have done my postdoctoral
fellowship at Novartis Corporation
where I investigated the functional differences between Raf kinase isoforms.
I have identified several novel human proteins some of which interact specifically
only with one Raf isoform. Two A-Raf specific proteins showed significant
sequence similarity to mitochondrial protein import receptors from lower
eukaryots by bioinformatics analysis. To further validate the biological
relevance of these interaction I found that A-Raf but not C-Raf protein
is concentrated in the rat liver mitochondria. I also found a novel
protein kinase interacting with all Raf isoforms. Its bioinformatics
analysis revealed that it most homologous to the dual specificity serine/threonine/tyrosine
kinases I have expressed this kinase in E.coli, purified it to homogeneity
for further biochemical characterization and X-ray crystallography.
In addition I have helped my supervisor Dr.
Lawrence P. Wennogle in starting the protein tyrosine phosphatase project
by cloning eight protein phosphatases. The goal of this project to
find protein tyrosine phosphatase inhibitors that block insulin-receptor
dephosphorylation and can be used as therapeutic drugs in type II diabetes.
For my extracurricular activity I have actively participated in the "Antisense
in Restenosis" project. As part of this effort I have supervised
two technicians and trained them in Molecular Biological techniques.
I am currently a project
team leader at deCODE Genetics Inc.
My group is developing a new strategy to find the mutations in the genomic
loci mapped by linkage analysis in human genome. The technology includes
amplification of large genomic fragments by long and accurate PCR, their
subcloning, high throughput sequencing and data analysis using proprietary
software. We are currently amplifying and cloning about 400 Kb of
human major histocompatibility region from chromosome 6 in order to search
for the mutations associated with various decease.