LOCUS 400272 431 aa 01-NOV-1997 DEFINITION MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44 PRECURSOR (MITOCHONDRIAL PROTEIN IMPORT PROTEIN 1) (INNER MEMBRANE IMPORT SITE PROTEIN 45) (ISP45) (MEMBRANE IMPORT MACHINERY PROTEIN MIM44). ACCESSION 400272 PID g400272 DBSOURCE SWISS-PROT: locus IM44_YEAST, accession Q01852 class: standard. created: Jul 1, 1993. sequence updated: Jul 1, 1993. annotation updated: Nov 1, 1997. xrefs: gi: 599967, gi: 599977, gi: 3966, gi: 3967, gi: 603997, gi: 763324, gi: 283201 xrefs (non-sequence databases): SGD L0001138 KEYWORDS MITOCHONDRION; INNER MEMBRANE; TRANSPORT; PROTEIN TRANSPORT; TRANSLOCATION; TRANSIT PEPTIDE; ATP-BINDING. SOURCE baker's yeast. ORGANISM Saccharomyces cerevisiae Eukaryotae; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. REFERENCE 1 (residues 1 to 431) AUTHORS Maarse,A.C., Blom,J., Grivell,L.A. and Meijer,M. TITLE MPI1, an essential gene encoding a mitochondrial membrane protein, is possibly involved in protein import into yeast mitochondria JOURNAL EMBO J. 11 (10), 3619-3628 (1992) MEDLINE 93010953 REMARK SEQUENCE FROM N.A. STRAIN=AB320 REFERENCE 2 (residues 1 to 431) AUTHORS BARRELL,B.G., BADCOCK,K., BANKIER,A.T., BOWMAN,S., BROWN,D., CHURCHER,C.M., CONNOR,R., COPSEY,T., DEAR,S., DEVLIN,K., FRASER,A., GENTLES,S., HAMLYN,N., HORSNELL,T.S., HUNT,S., JAGELS,K., JONES,M., LOUIS,E., LYE,G., MOULE,S., MOULE,T., ODELL,C., PEARSON,D., RAJANDREAM,M.A., RILES,L., ROWLEY,N., SKELTON,J., SMITH,V., WALSH,S.V. and WHITEHEAD,S. TITLE Direct Submission JOURNAL Submitted (??-DEC-1994) TO EMBL/GENBANK/DDBJ DATA BANKS REMARK SEQUENCE FROM N.A. STRAIN=S288C / AB972 REFERENCE 3 (residues 1 to 431) AUTHORS Horst,M., Jeno,P., Kronidou,N.G., Bolliger,L., Oppliger,W., Scherer,P., Manning-Krieg,U., Jascur,T. and Schatz,G. TITLE Protein import into yeast mitochondria: the inner membrane import site protein ISP45 is the MPI1 gene product JOURNAL EMBO J. 12 (8), 3035-3041 (1993) MEDLINE 93345448 REMARK PARTIAL SEQUENCE, AND CHARACTERIZATION. COMMENT [FUNCTION] INVOLVED IN PROTEIN IMPORT INTO THE MITOCHONDRION. PROBABLY INVOLVED IN TRANSLOCATION ACROSS THE INNER MEMBRANE. AS A BINDING PROTEIN REQUIRED FOR DRIVING THE IMPORT OF PREPROTEINS. RECRUITS MITOCHONDRIAL HSP70 AND ITS CO-CHAPERONE (MGE1) TO DRIVE PROTEIN TRANSLOCATION INTO THE MATRIX USING ATP AS AN ENERGY SOURCE. [SUBUNIT] FORMS PART OF THE RECEPTOR COMPLEX THAT CONSISTS OF AT LEAST 3 DIFFERENT PROTEINS (TIM17, TIM23, TIM44). [SUBCELLULAR LOCATION] MITOCHONDRIAL INNER MEMBRANE. FEATURES Location/Qualifiers source 1..431 /organism="Saccharomyces cerevisiae" /db_xref="taxon:4932" Region (1.430)..431 /note="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44." /region_name="Mature chain" Region 1..(2.431) /note="MITOCHONDRION." /region_name="Transit peptide" 1..431 Protein 1..431 /product="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44 PRECURSOR" Site 101..108 /note="ATP." /site_type="np-binding" ORIGIN 1 mhrstfirts gtssrtltar yrsqytgllv arvlfststt raqggnprsp lqifrdtfkk 61 eweksqelqe niktlqdasg klgeseaykk areaylkaqr gstivgktlk ktgetmehia 121 tkaweselgk ntrkaaaata kkldesfepv rqtkiykevs eviddgessr yggfitkeqr 181 rlkrerdlas gkrhravksn edagtavvat nieskesfgk kvedfkektv vgrsiqslkn 241 klwdesenpl ivvmrkitnk vggffaetes srvysqfklm dptfsnesft rhlreyivpe 301 ileayvkgdv kvlkkwfsea pfnvyaaqqk ifkeqdvyad grildirgve ivsakllapq 361 dipvlvvgcr aqeinlyrkk ktgeiaagde anilmssyam vftrdpeqid ddetegwkil 421 efvrggsrqf t //
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