LOCUS       125651        648 aa                              15-DEC-1998
DEFINITION  RAF PROTO-ONCOGENE SERINE/THREONINE-PROTEIN KINASE (RAF-1) (C-RAF).
ACCESSION   125651
PID         g125651
DBSOURCE    SWISS-PROT: locus KRAF_HUMAN, accession P04049
            class: standard.
            created: Nov 1, 1986.
            sequence updated: Nov 1, 1986.
            annotation updated: Dec 15, 1998.
            xrefs: gi: 35841, gi: 35842, gi: 66762
            xrefs (non-sequence databases): MIM 164760, PROSITE PS00107,
            PROSITE PS00108, PROSITE PS00479, PROSITE PS50011, PFAM PF00069,
            PFAM PF00130
KEYWORDS    TRANSFERASE; SERINE/THREONINE-PROTEIN KINASE; PROTO-ONCOGENE; ZINC;
            ATP-BINDING; PHORBOL-ESTER BINDING; 3D-STRUCTURE.
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Vertebrata; Mammalia; Eutheria;
            Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 648)
  AUTHORS   Bonner,T.I., Oppermann,H., Seeburg,P., Kerby,S.B., Gunnell,M.A.,
            Young,A.C. and Rapp,U.R.
  TITLE     The complete coding sequence of the human raf oncogene and the
            corresponding structure of the c-raf-1 gene
  JOURNAL   Nucleic Acids Res. 14 (2), 1009-1015 (1986)
  MEDLINE   86120351
  REMARK    SEQUENCE FROM N.A.
REFERENCE   2  (residues 1 to 648)
  AUTHORS   Nassar,N., Horn,G., Herrmann,C., Scherer,A., McCormick,F. and
            Wittinghofer,A.
  TITLE     The 2.2 A crystal structure of the Ras-binding domain of the
            serine/threonine kinase c-Raf1 in complex with Rap1A and a GTP
            analogue
  JOURNAL   Nature 375 (6532), 554-560 (1995)
  MEDLINE   95312074
  REMARK    X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 51-131.
REFERENCE   3  (residues 1 to 648)
  AUTHORS   Nassar,N., Horn,G., Herrmann,C., Block,C., Janknecht,R. and
            Wittinghofer,A.
  TITLE     Ras/Rap effector specificity determined by charge reversal
  JOURNAL   Nat. Struct. Biol. 3 (8), 723-729 (1996)
  MEDLINE   96313130
  REMARK    X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 56-131.
REFERENCE   4  (residues 1 to 648)
  AUTHORS   Emerson,S.D., Madison,V.S., Palermo,R.E., Waugh,D.S.,
            Scheffler,J.E., Tsao,K.L., Kiefer,S.E., Liu,S.P. and Fry,D.C.
  TITLE     Solution structure of the Ras-binding domain of c-Raf-1 and
            identification of its Ras interaction surface
  JOURNAL   Biochemistry 34 (21), 6911-6918 (1995)
  MEDLINE   95284022
  REMARK    STRUCTURE BY NMR OF 55-132.
REFERENCE   5  (residues 1 to 648)
  AUTHORS   Mott,H.R., Carpenter,J.W., Zhong,S., Ghosh,S., Bell,R.M. and
            Campbell,S.L.
  TITLE     The solution structure of the Raf-1 cysteine-rich domain: a novel
            ras and phospholipid binding site
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 93 (16), 8312-8317 (1996)
  MEDLINE   96323218
  REMARK    STRUCTURE BY NMR OF 136-187.
COMMENT     -------------------------------------------------------------------
            This SWISS-PROT entry is copyright. It is produced through a
            collaboration between the Swiss Institute of Bioinformatics and
            the EMBL outstation - the European Bioinformatics Institute.
            The original entry is available from http://www.expasy.ch/sprot
            and http://www.ebi.ac.uk/sprot
            ------------------------------------------------------------------.
            [FUNCTION] INVOLVED IN THE TRANSDUCTION OF MITOGENIC SIGNALS FROM
            THE CELL MEMBRANE TO THE NUCLEUS. PART OF THE RAS-DEPENDENT
            SIGNALLING PATHWAY FROM RECEPTORS TO THE NUCLEUS.
            [SIMILARITY] WITH THE CONSERVED CATALYTIC DOMAINS OF
            SER/THR-PROTEIN KINASES. BELONGS TO THE MIL/RAF SUBFAMILY.
            [SIMILARITY] CONTAINS A COPY OF THE ZINC-DEPENDENT PHORBOL-ESTER
            AND DAG BINDING DOMAIN.
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     1..648
     Protein         1..648
                     /product="RAF PROTO-ONCOGENE SERINE/THREONINE-PROTEIN
                     KINASE"
                     /EC_number="2.7.1.-"
     Region          139..184
                     /note="PHORBOL-ESTER AND DAG BINDING."
                     /region_name="Domain"
     Region          349..609
                     /note="PROTEIN KINASE."
                     /region_name="Domain"
     Site            355..363
                     /note="ATP."
                     /site_type="np-binding"
     Site            375
                     /note="ATP."
                     /site_type="binding"
     Site            468
                     /site_type="active"
ORIGIN      
        1 mehiqgawkt isngfgfkda vfdgsscisp tivqqfgyqr rasddgkltd psktsntirv
       61 flpnkqrtvv nvrngmslhd clmkalkvrg lqpeccavfr llhehkgkka rldwntdaas
      121 ligeelqvdf ldhvpltthn farktflkla fcdicqkfll ngfrcqtcgy kfhehcstkv
      181 ptmcvdwsni rqlllfpnst igdsgvpalp sltmrrmres vsrmpvssqh rystphaftf
      241 ntsspssegs lsqrqrstst pnvhmvsttl pvdsrmieda irshsesasp salssspnnl
      301 sptgwsqpkt pvpaqrerap vsgtqeknki rprgqrdssy yweieasevm lstrigsgsf
      361 gtvykgkwhg dvavkilkvv dptpeqfqaf rnevavlrkt rhvnillfmg ymtkdnlaiv
      421 tqwcegssly khlhvqetkf qmfqlidiar qtaqgmdylh akniihrdmk snniflhegl
      481 tvkigdfgla tvksrwsgsq qveqptgsvl wmapevirmq dnnpfsfqsd vysygivlye
      541 lmtgelpysh innrdqiifm vgrgyaspdl sklykncpka mkrlvadcvk kvkeerplfp
      601 qilssiellq hslpkinrsa sepslhraah tedinactlt tsprlpvf
//

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