LOCUS       1170701       765 aa                              15-DEC-1998
DEFINITION  B-RAF PROTO-ONCOGENE SERINE/THREONINE-PROTEIN KINASE (P94).
ACCESSION   1170701
PID         g1170701
DBSOURCE    SWISS-PROT: locus KRAB_HUMAN, accession P15056
            class: standard.
            created: Nov 1, 1990.
            sequence updated: Nov 1, 1995.
            annotation updated: Dec 15, 1998.
            xrefs: gi: 179532, gi: 179533, gi: 179534, gi: 179535, gi: 2144413,
            gi: 106860
            xrefs (non-sequence databases): MIM 164757, PROSITE PS00107,
            PROSITE PS00108, PROSITE PS50011, PROSITE PS00479, PFAM PF00069,
            PFAM PF00130, HSSP P04049
KEYWORDS    TRANSFERASE; SERINE/THREONINE-PROTEIN KINASE; PROTO-ONCOGENE; ZINC;
            ATP-BINDING; PHORBOL-ESTER BINDING; PHOSPHORYLATION.
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Vertebrata; Mammalia; Eutheria;
            Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 765)
  AUTHORS   Stephens,R.M., Sithanandam,G., Copeland,T.D., Kaplan,D.R.,
            Rapp,U.R. and Morrison,D.K.
  TITLE     95-kilodalton B-Raf serine/threonine kinase: identification of the
            protein and its major autophosphorylation site
  JOURNAL   Mol. Cell. Biol. 12 (9), 3733-3742 (1992)
  MEDLINE   92375040
  REMARK    SEQUENCE FROM N.A., AND PARTIAL SEQUENCE.
            TISSUE=TESTIS
REFERENCE   2  (residues 1 to 765)
  AUTHORS   Sithanandam,G., Kolch,W., Duh,F.M. and Rapp,U.R.
  TITLE     Complete coding sequence of a human B-raf cDNA and detection of
            B-raf protein kinase with isozyme specific antibodies
  JOURNAL   Oncogene 5 (12), 1775-1780 (1990)
  MEDLINE   91133728
  REMARK    SEQUENCE OF 116-765 FROM N.A.
            TISSUE=TESTIS
REFERENCE   3  (residues 1 to 765)
  AUTHORS   Ikawa,S., Fukui,M., Ueyama,Y., Tamaoki,N., Yamamoto,T. and
            Toyoshima,K.
  TITLE     B-raf, a new member of the raf family, is activated by DNA
            rearrangement
  JOURNAL   Mol. Cell. Biol. 8 (6), 2651-2654 (1988)
  MEDLINE   88302178
  REMARK    SEQUENCE OF 438-765 FROM N.A.
COMMENT     On Jan 29, 1996 this sequence version replaced gi:125648.
            -------------------------------------------------------------------
            This SWISS-PROT entry is copyright. It is produced through a
            collaboration between the Swiss Institute of Bioinformatics and
            the EMBL outstation - the European Bioinformatics Institute.
            The original entry is available from http://www.expasy.ch/sprot
            and http://www.ebi.ac.uk/sprot
            ------------------------------------------------------------------.
            [FUNCTION] INVOLVED IN THE TRANSDUCTION OF MITOGENIC SIGNALS FROM
            THE CELL MEMBRANE TO THE NUCLEUS.
            [FUNCTION] MAY PLAY A ROLE IN THE POSTSYNAPTIC RESPONSES OF
            HIPPOCAMPAL NEURON.
            [SUBCELLULAR LOCATION] CYTOPLASMIC.
            [TISSUE SPECIFICITY] CEREBRUM AND TESTES.
            [SIMILARITY] WITH THE CONSERVED CATALYTIC DOMAINS OF
            SER/THR-PROTEIN KINASES. BELONGS TO THE MIL/RAF SUBFAMILY.
            [SIMILARITY] CONTAINS A COPY OF THE ZINC-DEPENDENT PHORBOL-ESTER
            AND DAG BINDING DOMAIN.
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     1..765
     Protein         1..765
                     /product="B-RAF PROTO-ONCOGENE SERINE/THREONINE-PROTEIN
                     KINASE"
                     /EC_number="2.7.1.-"
     Region          6..11
                     /note="POLY-GLY."
                     /region_name="Domain"
     Region          121..128
                     /note="POLY-SER."
                     /region_name="Domain"
     Region          234..279
                     /note="PHORBOL-ESTER AND DAG BINDING."
                     /region_name="Domain"
     Site            372
                     /note="(AUTO-)."
                     /site_type="phosphorylation"
     Region          427..431
                     /note="POLY-SER."
                     /region_name="Domain"
     Region          456..716
                     /note="PROTEIN KINASE."
                     /region_name="Domain"
     Site            462..470
                     /note="ATP."
                     /site_type="np-binding"
     Site            482
                     /note="ATP."
                     /site_type="binding"
     Site            575
                     /site_type="active"
     Region          765
                     /note="H -> D (IN REF. 3)."
                     /region_name="Conflict"
ORIGIN      
        1 maalsggggg gaepgqalfn gdmepeagag rpaassaadp aipeevwnik qmikltqehi
       61 ealldkfgge hnppsiylea yeeytsklda lqqreqqlle slgngtdfsv sssasmdtvt
      121 sssssslsvl psslsvfqnp tdvarsnpks pqkpivrvfl pnkqrtvvpa rcgvtvrdsl
      181 kkalmmrgli peccavyriq dgekkpigwd tdiswltgee lhvevlenvp ltthnfvrkt
      241 fftlafcdfc rkllfqgfrc qtcgykfhqr cstevplmcv nydqldllfv skffehhpip
      301 qeeaslaeta ltsgsspsap asdsigpqil tspspsksip ipqpfrpade dhrnqfgqrd
      361 rsssapnvhi ntiepvnidd lirdqgfrgd ggsttglsat ppaslpgslt nvkalqkspg
      421 pqrerkssss sedrnrmktl grrdssddwe ipdgqitvgq rigsgsfgtv ykgkwhgdva
      481 vkmlnvtapt pqqlqafkne vgvlrktrhv nillfmgyst kpqlaivtqw cegsslyhhl
      541 hiietkfemi klidiarqta qgmdylhaks iihrdlksnn iflhedltvk igdfglatvk
      601 srwsgshqfe qlsgsilwma pevirmqdkn pysfqsdvya fgivlyelmt gqlpysninn
      661 rdqiifmvgr gylspdlskv rsncpkamkr lmaeclkkkr derplfpqil asiellarsl
      721 pkihrsasep slnragfqte dfslyacasp ktpiqaggyg afpvh
//

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