1995-1997:
Postdoctoral Research Training, Molecular and Cellular Biology Research
Unit,
Pharmaceutical Division, Novartis Corporation,
Summit, NJ USA
1991-1995:
Ph.D. in Molecular Biology and Genetics
Graduate Program in Biochemistry, Cellular and Molecular Biology,
Johns
Hopkins University, School of Medicine
, Baltimore, MD USA
Thesis: C-terminal Domain of RNA polymerase II: Some Insights
on its Function in vivo.
1987-1989:
MS in Cellular and Molecular Biology with Honors
Shemyakin’s
Institute of Bioorganic Chemistry, Academy of Sciences, Moscow,
USSR
Thesis: Isolation and Physical Mapping of Gene Encoding a2
isoform of Na,K-ATPase.
1983-1987:
BS in Physics and Biophysics with Honors
Moscow
Institute of Physics and Technology, Moscow, USSR. (about
PhysTech)
Senior Research Scientist. deCODE
Genetics Inc., Research and Development Group, Iceland.
ACCOMPLISHMENTS
Developing novel proprietary technologies in Molecular Biology
for high throughput amplification and sequencing of large regions
of human genome. The technology involves amplification of human
genomic DNA by long and accurate PCR, subcloning and shotgun sequencing.
I am also involved in organizing the high throughput functional analysis
of potential drug targets by two-hybrid screen.
9.1995-9.1997
Postdoctoral Research Scientist. Novartis
Corporation, Molecular and Cellular Biology Research Unit, Summit,
New Jersey, USA. Supervisor: Lawrence
P. Wennogle, Ph.D.
ACCOMPLISHMENTS
Studied protein kinase RAF isoform specific functions and mapped
the principal RAF protein-protein interaction domain. Identified RAF
isoform specific protein interactions by two-hybrid screen.
Discovered genes encoding human mitochondrial protein import receptors.
Demonstrated that A-RAF isoform is imported into mammalian mitochondria.
Expressed in E.coli and purify a novel growth associated mammalian protein
kinase, which interact with RAF cystein-rich domain in the two-hybrid assay.
Developed an in vitro biochemical assay for this kinase.
9.1991-7.1995
Graduate Student. Johns
Hopkins University, School of Medicine, Department
of Molecular Biology
and
Genetics, Baltimore, Maryland, USA. Supervisor: Jeffry
L. Corden, Ph.D.
ACCOMPLISHMENTS
Studied the function of yeast RNA polymerase II C-terminal domain (CTD)
using methods of yeast genetics. Developed the selection system for
mutant hunt, cloned a novel suppressor gene. Analyzed by suppression
analysis lethal mutations.
Developed the method to search for CTD interacting proteins by two-hybrid
screen and discovered a novel family of splicing-related proteins that
interact with CTD of RNA polymerase II using two-hybrid screen method.
This discovery suggested the existence of the physical link between transcription
and splicing in eukaryotic cells.
1.1992-3.1992
Graduate Student. Johns Hopkins University, School of Medicine,
Department of Molecular Biology and Genetics
Rotation project. Supervisor: Daniel
Nathans, M.D.
ACCOMPLISHMENTS
Phosphopeptide mapping of JunB protein.
9.1990-9.1991
Senior Laboratory Assistant. Johns Hopkins University, School
of Medicine, Rheumatology Division. Supervisor: Joseph
M. Ahearn, M.D.
ACCOMPLISHMENTS
Participated in the determination of the structural basis for selective
binding of Epstein-Barr virus (EBV) to human complement receptor type 2
(CR2) using methods of molecular cloning and tissue culture. The
parts of human CR2 receptor were substituted by mouse CR2 and mouse-human
CR2 chimeras were assayed for their ability to bind (EBV) using methods
of tissue culture transfection or infection with retroviruses.
11.1988 - 9.1990
Research Associate. Shemyakin's
Institute of Bioorganic Chemistry, Academy of Sciences, Moscow, USSR
9/1989 - 9/1990 RESEARCH ASSOCIATE, Supervisor: Prof.
N. N. Modyanov
Isolated and physically mapped the 5’-end of the gene, encoding a1
isoform of Na,K-ATPase
11/1988-9/1989 SENIOR LABORATORY ASSISTANT, Supervisor:
Prof.
N. N. Modyanov
ACCOMPLISHMENTS
Isolated and physically mapped genes encoding different Na,K-ATPase isoforms.
5.1998
- present
